Single-Dilution Enzyme-Linked Immunosorbent Assay for Quantification of Antigen-Specific Salmonid Antibody
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چکیده
منابع مشابه
Single-dilution enzyme-linked immunosorbent assay for quantification of antigen-specific salmonid antibody.
An enzyme-linked immunosorbent assay (ELISA) was developed on the basis of testing a single dilution of serum to quantify the level of antibody to the p57 protein of Renibacterium salmoninarum in sockeye salmon (Oncorhynchus nerka). The levels of antibody were interpolated from a standard curve constructed by relating the optical densities (OD) produced by several dilutions of a high-titer rain...
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An enzyme-linked immunosorbent assay for neopterin using penicillinase as marker enzyme is reported here by polyclonal antibodies against neopterin conjugated to bovine serum albumin which were raised in rabbits. Immunoglobulin fractions were purified and coated on wells of microtiter plates. A chain heterology was introduced in neopterin derivative and conjugated to penicillinase. The assa...
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ژورنال
عنوان ژورنال: Journal of Veterinary Diagnostic Investigation
سال: 2000
ISSN: 1040-6387,1943-4936
DOI: 10.1177/104063870001200308